Dr. Mouton’s Biosketch

Biosketch &
Curriculum Vitae

Peter R. Mouton, Ph.D.
CEO & Chief Scientific Officer
SRC Biosciences (Stereology Resource Center, Inc.)

1810 W. Kennedy Blvd
Tampa, FL, USA

Education

  • University of South Florida, College of Natural Sciences, Tampa, FL - Bachelor Degree (B.S. Biology), 1983
  • University of South Florida, College of Natural Sciences, Tampa, FL - Bachelor Degree (B.A. Chemistry), 1983
  • Department of Neurobiology, Karolinska Institute, Stockholm, Sweden - Predoctoral Fellowship in Neurobiology, 1987-1988 (Prof. dr. Lars Olson)
  • University of South Florida, Colleges of Medicine and Natural Sciences, Tampa, FL - Ph. D. in Neurosciences, 1990
  • Laboratory of Stereology and Neurology, University of Copenhagen, Denmark - Postdoctoral Fellowship in Stereology, 1990-1992 (Prof. dr. Hans J. G. Gundersen)
  • The Johns Hopkins Medical School, Department of Pathology, Baltimore, MD - Postdoctoral Fellowship in Neuropathology, 1992-1994. (Prof. Donald L. Price, M.D.).

Professional Training

Dr. Mouton’s professional training includes a one-year predoctoral fellowship in neurobiology at the Karolinska Institute (Stockholm, Sweden) and Ph.D. in Neuroscience from the University of South Florida (USF, Tampa, FL), a two-year postdoctoral stereology fellowship in Denmark with Professor Hans J.G. Gundersen, the “father of unbiased stereology,” followed by a two-year NIH fellowship in Neuropathology with Professor Donald L. Price at the Pathology Department at the Johns Hopkins University School of Medicine (Baltimore MD). He subsequently served on faculty at the Hopkins Pathology Department for six years before establishing the Neurostereology of Brain Aging group at the Hopkins Bayview/National Institute on Aging campus in Baltimore. His stereology work in autopsied human brains was the first to 1) stability of noradrenergic neurons in locus coeruleus during normal brain aging; 2) substantial neuron loss in hypothalamic regions from patients with major depression; and, the strongest correlations to date between neocortical degeneration and progressive dementia in Alzheimer’s disease. Other stereology projects during this time were the first stereology protocols for analyzing human and animal brain aging and autism and quantification of the earliest mouse models expressing human mutations for Alzheimer’s, Parkinson’s and other neurodegenerative diseases.

Contributions to Science

In parallel with his academic studies, Dr. Mouton’s leadership of the SRC Biosciences has provided the global community of bioscientists in academia, government agencies and preclinical pharmacology research groups with powerful technology for their stereology studies. Commercialization of the Stereologer system, the first fully integrated hardware-software-microscopy stereology system, led to an exponential increase in neuroscience stereology studies during the decade following its introduction in the mid 1990s. To date this system has supported thousands of stereology studies across all the neuroscience sub-disciplines from quantifying gross morphology to cellular and synapse counts at the resolving limit of the light microscope [a]. During the past two decades he developed and commercialized the Stereologer™, the only computerized stereology to receive innovation awards from both the National Science Foundation and the National Institutes of Health. Since the mid-1990s, the SRC’s contract research organization has provided the international bioscience community with professional stereology services and consultation. In 2010 Dr. Mouton returned to USF in Tampa where he continues to serve on faculty as Professor of Stereology and Chief Scientific Officer of SRC Biosciences.

Current Positions

  • 2003-present: President & Chief Scientific Officer, SRC Biosciences (Stereology Resource Center, Inc.), 1810 W. Kennedy Blvd, Tampa, FL
  • 2010-present: Professor of Stereology & Computer Sciences, College of Engineering, University of South Florida, Tampa, FL (Courtesy Appointment).
  • 2016-present: Standing Study Section Member: ETTN-H (11B ), Drug Discovery for Aging, Neuropsychiatric and Neurological Diseases. NIH Center for Scientific Review.
  • 2017-present: Member Editorial Board, Journal of Chemical Neuroanatomy
  • 2018-present. Professorem Hospitem (Visiting Professor), 4EU Alliance, Charles University at Prague, Czech Republic.
  • 2018-2021: Fulbright Specialist, Bureau of Education & Cultural Affairs, U.S. Department of State.
  • 2020: Fellow & Member of Education Committee, Digital Pathology Association.

Past Positions

  • Director Stereology Core Facility, Gerontology Research Center, National Institute on Aging, Baltimore, MD.
  • Assistant Professor, Faculty in Pathology Department, The Johns Hopkins University School of Medicine, Baltimore, MD.

Grant Awards & Contracts

SRC Biosciences is funded by research grants and outsourcing contracts to study the biological basis of diseases; provide training to individuals and groups; and, develop technology to enhance health and well-being. Proprietary information outside the public domain about CRO contracts with academic researchers, government agencies and private industry is not listed below.

National Science Foundation (NSF)

Project Title: Microscope-based Technology For Automatic Cell Analysis Using Unbiased Methods.
Project Role: Peter R. Mouton, Principal Investigator
Award Type: STTR Phase II Award: NSF #1926990
Duration: 01 September 2019 – 31 August 2021
Project Goal: Develop and validate deep learning neural networks for automatic stereology of biological structure on stained tissue sections.

National Institute on Aging (NIH)

Project Title: Treating with Gamma-Secretase Modulators to Prevent
Project Role: Peter R. Mouton, Principal Investigator, Neuropathology Subaward
Award Type: R01 National Institute on Aging (Mobley, William, PI, 1R01AG055523-01A1).
Duration: 01 May 2018 – 31 January 2023
Project Aim: 1. Detail the time of onset of neurodegeneration in the Dp16 model of AD-DS and Line 41 AD model using unbiased stereology. 2. Examine the effect of BPN15606 treatment before and after onset of neurodegeneration in the AD-DS model, the AD/cerebral amyloidosis model and the Line 41 mouse model.

National Institute on Aging (NIH)

Project Title: Antisense Oligonucleotides Targeting APP to Prevent Neurodegeneration in Models of Down Syndrome and Alzheimer’s disease.
Project Role: Peter R. Mouton, Principal Investigator, Neuropathology Subaward
Award Type: R01 National Institute on Aging (Mobley, William, PI, 1R01AG061151-01)
Duration: 01 September 2018 to 31 August 2023
Project Goal: Assess the ability of ASO targeting to mitigate AD neuropathology in mouse models of AD and Down syndrome.

Florida High Tech Corridor Grant Program

Project Title: Applications of Deep Learning and Automatic Stereology to Assess Treatments for Alzheimer’s Disease.
Project Role: Co-PIs (Mouton/Goldgof))
Duration: 01 September 1, 2019 – August 31, 2021
Project Goal: Apply artificial intelligence to stereology analysis of Alzheimer’s disease neuropathology.

U.S. Department of State

Project Title: Fulbright U.S. Scholar Award
Project Role: Principal Investigator
Duration: 09 April 2018 – 08 April 2021
Project Goal: Provide the international bioscience community with professional stereology support through on-site training, collaboration and consultation.

National Science Foundation (NSF)

Project Title: Support for Fall 2019 NSF I-Corps Workshop (USF, Tampa, FL).
Project Role: Principal Investigator
Award Type: STTR Phase II Award: NSF #1926990
Duration: September-November 2019 (6 weeks).
Project Goal: Workshop on team-based customer discovery to commercialize biotechnology for the global bioscience community.

Selected Publications (> 100 total)

Peer-reviewed Journals

  • Řimnáčová, H., Štiavnická, M., Moravec, J., Chemek, M., Kolinko, Y., García-Álvarez, O., Peter R. Mouton, Trejo, A.M.C., Fenclová, T., Eretová, N., Hošek, P., Klein, P., Králičková, M., Nevoral, J. Post-translational protein modifications as a mode of endocrine disrupting action of bisphenol S in male reproduction. Reproductive Toxicology, in review.
  • Walters, K., Boucher, M., Boucher, G.G., Opsahl, A.C., Mouton, P.R., Liu, C.-N., Ritenour, C.R., Kawabe, T.T., Pryski, H.N., Somps, C.J. No evidence of neurogenesis in adult rat sympathetic ganglia following guanethidine-induced neuronal necrosis, Toxicologic Path, 48(1):228-237, 2020.
  • Goulding, D., Kraft, A., Peter R. Mouton, McPherson, C.A., Avdoshina, V., Mocchetti, I., Harry, G.J. Age-related decrease in tyrosine hydroxylase immunoreactivity in the substantia nigra and region-specific changes in microglia morphology in HIV-1 Tg rats. Neurotoxicology Research July 8, 2019. doi: 10.1007/s12640-019-00077.
  • Klebe, D., Tribewal, M., Vanaparthy, R., Varghese, M., Cheng, B., Peter R. Mouton, Velishek, J., Dobrenis, K., Hof, P.R., and Ballabh, P. Reduced hippocampal dendrite branch, spine density and cognitive function in preterm rabbits and reversal with estrogen or TrkB agonist. Cerebral Cortex, March 16, 2019. doi: 10.1093/cercor/bhz033
  • Phoulady, H.A., Goldgof, D., Lawrence O. Hall, L.O., Peter R. Mouton. Automatic Ground Truth for Deep Learning Stereology of Immunostained Neurons and Microglia in Mouse Neocortex. J. Chemical Neuroanatomy, 96: 1-7, 2019. doi: 10.1016/j.jchemneu.2019.02.00.
  • Alahmari, S., Goldgof, D., Hall, D. Phoulady, H.A. Patel, R., Peter R. Mouton. Automated Counts of Stained Cells by Deep Learning and Unbiased Stereology. J Chem Neuroanat, 96:94-101, 2019. doi: 10.1016/j.jchemneu.2018.12.010.
  • Phoulady, H.A., Goldgof, D., Hall, L.O., Nash, K.N., Peter R. Mouton. Automatic Stereology of Mean Nuclear Size of Neurons using an Active Contour Framework. J Chem Neuroanat, 96:110-115, 2019. doi: 10.1016/j.jchemneu.2018.12.012.
  • Peter R. Mouton, Phoulady, H.A., Goldgof, D., Hall, L.O., Gordon, M., Morgan, D. Unbiased estimation of cell number using the automatic optical fractionator. J Chem Neuroanat, 80:1-8, 2017. doi: 10.1016/j.jchemneu.2018.12.012.
  • Phoulady, H.A., Goldgof, D.B., Hall, L.O., Peter R. Mouton. A framework for nucleus and overlapping cytoplasm segmentation in cervical cytology extended depth of field and volume images. Computerized Medical Imaging and Graphics, Volume 59, Pages 38-49, July 2017.
  • Peter R. Mouton. Unbiased Stereology Of Neural Systems: From Normal Aging To Mental Illness And Neurodegenerative Diseases (Editor). Special issue, J Chem Neuroanat, Volume 76, 2016.
  • Marcek, J., Okerberg, C., Liu, C.N., Potter D., Butler, P., Boucher, M., Peter R. Mouton, Nyengaard, J.R., Somps, C. Anti-NGF monoclonal antibody mu Mab 911 does not deplete neurons in the superior cervical ganglia of young or old adult rats. J Chem Neuroanat. 76: 133 2016.
  • Weise, C. M., Peter R. Mouton, Eschbacher, J., Coons, S. W., Krakoff, J. A Post-Mortem Stereological Study Of Striatal Cell Number In Human Obesity. Obesity, 23(1):100-4, 2015.
  • Peter R. Mouton, Sobin, C. Developing a census of brain cells for the corticoendogram of human consciousness. J Chem Neuroanatomy, 2015.
  • Harry, G.J., Hooth, M.J., Vallant, M., Behl, M., Travlos, G.S., Howard, J.L., Price, C.J., McBride, S., Mervis, R., Peter R. Mouton, Neurodevelopmental Effects Of Gestational And Perinatal Thyroid Disruption In Rats. Toxics, 2, 496-532, 2014.
  • Stoner, R., Chow, M.L., Boyle, M.P., Sunkin, S.M., Peter R. Mouton, Roy, S., Wynshaw-Boris, A., Colamarino, S., Lein, E.S., Courchesne, E. Discrete Patches of Laminar Disorganization in Young Autistic Neocortex. New England Journal of Medicine, January, 2014.
  • Manaye, K.F., Peter R. Moutonn, Xu, G., Drew, A., Lei, D-L, Yukti, S., Rebeck, G.W., Turner, S., Age-related loss of noradrenergic neurons in the brains of triple transgenic mice. AGE 35: 139-147, 2013.
  • Selenica, M-L. B., Alvarez, J.A., Nash, K.R., Lee, D.C., Cao, C., Lin, X., Reid, P., Peter R. Mouton, Morgan, D., Gordon, M.N. Diverse Activation Of Microglia By Chemokine (C-C Motif) Ligand 2 Overexpression In Brain. Journal Of Neuroinflammation, 10:86, 2013.
  • Elozory, D.T., Bonam, O.P., Kramer, K., Goldgof, D., Hall, L., Mangual, O., Peter R. Mouton, Automatic Section Thickness Determination Using An Absolute Gradient Focus Function. J Microsc, 248(3): 245-59, 2012.
  • Peter R. Mouton, Kelley-Bell, B., Tweedie, D., Spangler, E., Perez, E., Carlson, O.D., Short, R.G., Decabo, R., Chang, J., Ingram, D.K., Li, Y., Greig, N.H. Effects Of Age And LPS-Mediated Inflammation On Central Catecholaminergic Neuron Number In B6 Mice. Neurobiology Of Aging, 423:27-36, 2012.
  • Manaye, K.F., Peter R. Mouton, Xu, G., Drew, A., Lei, D-L, Yukti, S., Rebeck, G.W., Turner, S., Alzheimer’s Type Neuron Loss In Locus Coeruleus Of Triple Transgenic Mice. AGE, November 30, 2011.
  • Courchesne, E., Peter R. Mouton, Calhoun, M.E., Semendeferi, K., Ahrens-Barbeau, C., Carter, C., Pierce, K. Neuron Number And Size In Prefrontal Cortex Of Children With Autism. JAMA, 306 (18):2001-10, 2011.
  • Manaye, K.F., Kalifa, S., Drew, A.C., Edossa, A., Lei, D-L, Ingram, D.K., Peter R. Mouton. 17α-estradiol attenuates neuron loss in ovariectomized Dtg AβPP/PS1 mice. Journal of Alzheimer’s Disease, 23:629-39, December 2010.
  • Peter R. Mouton, Chachich, M.E., Quigley, C., Spangler, E., Ingram, D.K. Caloric Restriction Attenuates Cortical Amyloidosis In A Double Transgenic Mouse Model Of Alzheimer’s Disease. Neuroscience Lett 464:184-7, 2009.
  • Burke, M, Zangenehpour, S, Peter R. Mouton, Ptito, M. Knowing What Counts: Unbiased Stereology In The Non-Human Primate Brain. J Vis Exp. 2009 May 14; (27) 1262-1266. Video: http://www.Jove.Com/Index/Details.Stp?ID=1262.
  • Duffy, K.B, Spangler E.L., Devan B.D., Guo Z., Bowker J.L., Janas, A.M., Hagepanos, A., Minor, R.K., Decabo R., Peter R. Mouton, Shukitt-Hale, B., Joseph, J.A., Ingram, D.K. A Blueberry-Enriched Diet Provides Cellular Protection Against Oxidative Stress And Reduces A Kainate-Induced Learning Impairment In Rats. Neurobiol Aging. 29:1680-9, 2008.
  • Manaye, K.F., Wang, P., O’Neil, J., Huafu, S., Tizabi, Y., Thompson, N., Ottinger, M.A., Ingram, D.K., Peter R. Mouton. Neuropathological Quantification Of Dtg APP/PS1. AGE 29:87-96, 2007.
  • O’Neil, J.N., Peter R. Mouton, Tizabi Y., Ottinger, M.A., Lei, D-L., Ingram, D.K., Manaye, K.F. Catecholaminergic Neuron Number In Locus Coeruleus Of Aged Female Dtg App/Ps1 Mice. J. Chem.Neuroanat. 34(3-4): 102-7, 2007.
  • Perry, T.A., Holloway, H.W., Weerasuriya, A., Peter R. Mouton, Duffy, K., Mattison J.A. Evidence Of GLP-1-Mediated Neuroprotection In An Animal Model Of Pyridoxine-Induced Peripheral Sensory Neuropathy. Exp Neurol 203:293-301, 2007.

Peer-reviewed Books & Book Chapters

Invited Peer-reviewed Conference Papers

  • Alahmari, S., Goldgof, D., Hall, D. Dave, P., Phoulady, H.A. Patel, R., Peter R. Mouton. Iterative Deep Learning Based Unbiased Stereology With Human-in-the-Loop. 17th IEEE International Conference on Machine Learning and Applications, Orlando, Florida, December 17-20, 2018.
  • Phoulady, H. A., Mouton, P.R. New cervical cytology dataset for nucleus detection and image classification (Cervix93) and methods for cervical nucleus detection. Proc. IEEE International Symposium on Biomedical Imaging, November, 2018.
  • Phoulady, H.A., Goldgof, D., Hall, L.O., Peter R. Mouton Histopathology Image Segmentation with Hierarchical Multilevel Thresholding, In Proceedings of the SPIE Medical Imaging on Digital Pathology, San Diego, CA, 2016.
  • Phoulady, H. A., Peter R. Mouton. New cervical cytology dataset for nucleus detection and image classification (Cervix93) and methods for cervical nucleus detection. IEEE International Symposium on Biomedical Imaging, arXiv:1811.09651, November 23, 2018.
  • Phoulady, H.A., Goldgof, D.B., Hall, L.O., Peter R. Moutonn. A Framework for Nucleus and Overlapping Cytoplasm Segmentation in Cervical Cytology Extended Depth of Field and Volume Images. Computerized Medical Imaging and Graphics, 59: 38-49, 2017.
  • Peter R. Mouton, Ahmari, S.A., Phoulady, H.A., Hall, L.O., Goldgof, D.B. High-Speed Stereology Analysis Of Neural Structures By Fully Automatic Stereology Technology (FAST). 50th International Congress of the Czech Anatomical Society, Pilsen, Czech Republic, September 2017.
  • Phoulady, H.A., Goldgof, D.B., Hall, L.O., and Peter R. Mouton. A new approach to detect and segment overlapping cells in multi-layer cervical cell volume images. Proceedings of the International Symposium on Biomedical Imaging (IEEE), Prague, Czech Republic, April 2016.
  • Chaudhury, B., Kramer, K., Elozory, D., Hernandez, G., Goldgof, D., Hall, L.O., Peter R. Mouton. A Novel Algorithm For Automated Counting Of Stained Cells On Thick Tissue Sections. In 25th International Symposium on Computer-based Medical Systems (CBMS) (Rome, Italy), pp. 1-6. IEEE, 2012.
  • Bonam, O., Elozory, D., Kramer, K., Goldgof, D., Hall, L.O., Mangual, O. Peter R. Mouton. Toward automated quantification of biological microstructures using unbiased stereology, Proc. SPIE, vol.7963, 2011.

Selected Awards, Honors, Patents (since 1997)

Intellectual Property

  • 1995 - Present: Inventor, Stereologer™, an Integrated Hardware-Software System for Unbiased Stereology Analysis.
  • 2015: Patent Stereology Software, Automatic Stereology System and Method For Automated Stereology of Cancer (International and U.S. Patent Serial No. 62/040,748).
  • 2016: Patent Stereology Software, Automatic Stereological Analysis of Biological Tissue Including Section Thickness Determination. United States Patent 9,297,995 B2. March 29, 2016.
  • Patent application filed for Stereology Software; Automated Stereology With Deep Learning To Quantify Biological Tissue. Patent filed April 2017.