Biosciences

Collaborations

 SRC Biosciences is part of the “High Tech I-4 Corridor” that spans central Florida along Interstate 4 that connects Tampa, Orlando and Gainesville. We collaborate within the consortium on proposals for competitive funding for neuroscience research and technology development from international, federal and state institutions. These collaborations provide a resource of high-tech industry in support of medical discoveries, scientific breakthroughs and drug development for improving health at the local, national and global levels.

Aerial view of Tampa, Florida

Funding for Innovative Technology

Intellectual Property

SRC Biosciences participates in IP-sharing of co-developed technology with USF, one of the nation’s top universities for technology transfer and commercialization.
  • “Automatic Stereological Analysis Of Biological Tissue Including Automatic Section Thickness Determination,” including an addendum, “Automated Stereology For Determining Tissue Characteristics,” Patent No. 9,297,995 B2, awarded Mar. 29, 2016.
  • “System And Method For Automated Stereology Of Cancer,” Patent WO 2016/029208-AI No. 62/040,748, awarded February 25, 2017.
  • “An Automatic Stereology Framework For Neuron Number Using The Automatic Optical Fractionator.” Patent Pending Applications, U.S. and International, Serial No. 62/420,771.

Publications with Stereologer Data

For more than two decades SRC Biosciences has led the stereology industry with innovative computerized systems (Stereologer®), professional training and efficient study designs for the global bioscience community.
autism brain diagrams
From Courchesne, E., Mouton, P.R., Calhoun, M.E., et al., Neuron Number And Size In Prefrontal Cortex Of Children With Autism. Journal Of The American Medical Association (JAMA), 306 (18):2001-10, November 9, 2011.

Peer Reviewed Stereologer Publications (sample of ~60 from over 3,000+ since 1996)

  • Mouton, P.R., et al., Effects Of Age And LPS-Mediated Inflammation On Central Catecholaminergic Neuron Number In B6 Mice. Neurobiology Of Aging, 423:27-36, 2012.
  • Alahmari, S., et al., Automated Counts Of Stained Cells By Deep Learning And Unbiased Stereology. J Chem Neuroanat, 96: 94-101, 2019.
  • Stoner, R, et al., Discrete Patches Of Laminar Disorganization In Young Autistic Neocortex, New England Journal Of Medicine, January 17, 2014.
  • Řimnáčová, H., et al., Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice. Reproductive Biology and Endocrinology (2020) 18:56, 2020.
  • Long JM, et al., What Counts In Brain Aging? Design-Based Stereological Analysis Of Cell Number. J Gerontology 54A: B407-B417, 1999.
  • Mouton PR. Principles And Practices Of Unbiased Stereology: An Introduction For Bioscientists. The Johns Hopkins University Press, Baltimore, 2002.
  • Manaye KF, et al., Selective Cell Loss In The Hypothalamus Of Patients Suffering From Depression. J Neuropath Exp Neurol 64:224-9, 2005.
  • Phoulady, H.A., et al., Automatic Ground Truth For Deep Learning Stereology Of Immunostained Neurons And Microglia In Mouse Neocortex. J Chem Neuroanat, 98: 1-7, 2019.
  • Mouton, P.R., et al., Unbiased Estimation Of Cell Number Using The Automatic Optical Fractionator.  J Chemical Neuroanat. 80:1-8, 2017.
  • Klebe, D., et al., Reduced Hippocampal Dendrite Branch, Spine Density And Cognitive Function In Preterm Rabbits And Reversal With Estrogen Or Trkb Agonist. Cerebral Cortex, March 16, 2019
  • O’Neil, J.N., Mouton, P.R., Tizabi Y., Ottinger, M.A., Lei, D-L., Ingram, D.K., Manaye, K.F. Catecholaminergic Neuron Number In Locus Coeruleus Of Aged Female Dtg App/Ps1 Mice. J. Chem.Neuroanat. 34(3-4): 102-7, 2007.
  • Mouton PR, et al., Cognitive Decline Strongly Correlates With Cortical Atrophy In Alzheimer’s Dementia. Neurobiol Aging 19: 371-377, 1998.
  • Scheff, S, et al., Synapse Stability In The Precuneus Early In The Progression Of Alzheimer’s Disease. J Alzheimers Dis. 24:547-57, 2013.
  • Francis HW, et al., Efficient Quantification Of Afferent Cochlear Ultrastructure Using Design-Based Stereology. J Neurosci Methods.150: 150-8, 2006.
  • Harry, G.J., et al., Neurodevelopmental Effects Of Gestational And Perinatal Thyroid Disruption In Rats. Toxics, 2, 496-532, 2014
  • Mouton, PR, et al., Developing A Census Of Brain Cells For The Corticoendogram Of Human Consciousness. J Chemical Neuroanat. 2015 76: 61, 2015.
  • Gemmell, E, et al., Hippocampal Neuronal Atrophy And Cognitive Function In Delayed Poststroke And Again-Related Dementias. Stroke. 43:808-14, 2012.
  • Mouton PR, et al., Stereological Length Estimation Using Spherical Probes. J Microsc 206: 54-64, 2002.
  • Kumar, A, et al., Traumatic Brain Injury In Aged Animals Increases Lesion Size And Chronically Alters Microglial/Macrophage Classical And Alternative Activation States. Neurobiol Aging. 34:1397-411, 2013.
  • Arnold, SA, et al., Serotonin 1A Receptor Agonist Increases Species-And Region-Selective Adult CNS Proliferation, But Not Through CNTF. Neuropharmacology. 63:1238-47, 2012.
  • Mouton, PR. Unbiased Stereology: A Concise Guide. The Johns Hopkins University Press, Baltimore, 2011.
  • Bakkum, BW, et al., Hetereogeneity Of Dose And Time Effects Of Estrogen On Neuron-Specific Neuronal Protein And Phosphorylated Cyclic AMP Response Element-Binding Protein In The Hippocampus Of Ovariectomized Rats. J Neurosci Res. 89:883-97, 2011.
  • Mouton PR, et al., Age And Gender Effects On Microglia And Astrocyte Number In Brains Of Mice. Brain Res 956:30-35, 2002
  • Lei D-L, et al., Effects Of Estrogen And Raloxifene On Neuroglia Number And Morphology In The Hippocampus Of Aged Female Mice. Neuroscience 121:659-666, 2003.
  • Mouton, PR. Applications Of Unbiased Stereology To Neurodevelopmental Toxicology, In Developmental Neurotoxicology Research: Principles, Models, Techniques, Strategies And Mechanisms (C. Wang And W. Slikke, Eds), John Wiley & Sons, Hoboken, N.J. Pg. 53-77, 2011b.
  • Healy-Stoffel, M, et al., Altered Nucleolar Morphology In Substantia Nigra Dopamine Neurons Following 6-Hydroxydopamine Lesion In Rats. Neurosci Lett. 546:26-30, 2013.
  • Berman, RF, et al., Low Level Neonatal Thimerosal Exposure: Further Evaluation Of Altered Neurotoxic Potential In SJL Mice. Toxicol. Sci. 101(2): 294-309, 2008.
  • Burke, M, et al., Knowing What Counts: Unbiased Stereology In The Non-Human Primate Brain. J Vis Exp. 14;(27) 2009.
  • Burke, M, et al., Neuronal Reduction In Frontal Cortex Of Primates After Prenatal Alcohol Exposure. Neuroreport. 20:13-7, 2009.
  • Calhoun ME, et al., Comparative Evaluation Of Synaptophysin-Based Methods For Quantification Of Synapses. J Neurocytol 25: 821-828, 1996.
  • Marcario JK, et al., Severe Subcortical Degeneration In Macaques Infected With Neurovirulent Simian Immunodeficiency Virus. J Neurovirol 10:1-13, 2004.
  • Calhoun ME, et al., Hippocampal Neuron And Synaptic Bouton Number In Aging C57bl/6 Mice. Neurobiol Aging 19: 599-606, 1998.
  • Chen-Roetlling, J, et al., A Rapid Fluorescent Method To Quantify Neuronal Loss After Experimental Intracerebral Hemorrhage. J Neurosci Methods. 15:216(2):128-36, 2013.
  • Craggs, LJ, et al., White Matter Pathology And Disconnection In The Frontal Lobe In CASASIL. Neuropathol Appl Neurobiol. July 12, 2013.
  • Dummula, K, et al., Bone Morphogenetic Protein Inhibition Protects After Intraventricular Hemorrhage. J. Neurosci. Aug 24;31(34):12068-12082, 2011.
  • Elozory, DT, et al., Automatic Section Thickness Determination Using An Absolute Gradient Focus Function. J Microscopy, 248(3): 245-59, 2012.
  • Gokhale, AM, et al., Stereological Estimation Of Surface Area In Thick Transparent Sections Of Arbitrary Orientation Using Virtual Cycloids. J Microsc 216(Pt1): 25-31, 2004.
  • Goti, D, et al., A Mutant Ataxin-3 Putative-Cleavage Fragment In Brain Of Machado-Joseph Disease Patients And Transgenic Mice Is Cytotoxic Above A Critical Concentration. J Neurosci 24:10266-79, 2004.
  • Hale, SL, et al., Modern Pathology Methods For Neural Investigations. Toxicol Pathol. 39:52-7, 2011.
  • Healy-Stoffel, M, et al., A Novel Use Of Combined Tyrosine Hydroxylase And Silver Nucleolar Staining To Determine The Effects Of A Unilateral Intrastriatal 6-Hydroxydopamine Lesion In The Substantia Nigra: A Stereological Study. J Neurosci Methods. 210(2):187-94, 2012.
  • Kane, CJ, et al., Protection Of Neurons And Microglia Against Ethanol In A Mouse Model Of Fetal Alcohol Spectrum Disorders By Peroxisome Proliferator-Activated Receptor-Y Agonists. Brain Behav Immun. 25 Suppl 1:S137-45, 2011.
  • Khundakar, A, et al., Cellular Pathology Within The Anterior Cingulate Cortex Of Patients With Late-Life Depression: A Morphometric Study. Psychiatry Res. 194:184-9, 2011.
  • Lee GD, et al., Stereological Analysis Of Microvasculature Parameters In A Double Transgenic Model Of Alzheimer’s Disease. Brain Res Bull 65:317-322, 2005.
  • Long JM, et al., Stereological Analysis Of Astrocyte And Microglia In Aging Mouse Hippocampus. Neurobiol Aging 19:497-503, 1999.
  • Mouton, P.R. Unbiased Stereology Of Neural Systems: From Normal Aging To Mental Illness And Neurodegenerative Diseases, Journal of Chemical Neuroanatomy, Vol 76, pp. 61-142, 2015.
  • Luo, W, et al., Inhibition Of Accelerated Graft Arteriosclerosis By Genetransfer Of Soluable Fgf Receptor-1 In Rat Aortic Transplants. Arterio Thrombosis Vascular Biology, 24:1081-6, 2004.
  • Manaye, KF, et al., Alzheimer’s Type Neuron Loss In Locus Coeruleus Of Triple Transgenic Mice. AGE, November 30, 2011.
  • Marcek J., et al., Anti-NGF Monoclonal Antibody Mu Mab 911 Does Not Deplete Neurons In The Superior Cervical Ganglia Of Young Or Old Adult Rats. J Chem Neuroanat. 76: 133 2016.
  • Maswood, N, et al., Caloric Restriction Stimulates Production Of Neurotrophic Factors And Attenuates Neurochemical And Behavioral Deficits In A Primate Model Of Parkinson Disease. Proc. Natl. Acad. Sci. Usa 101(52):18171-6, 2004.
  • Mooney,  SJ, et al., Social Condition And Oxytocin Neuron Number In The Hypothalamus Of Naked Mole-Rats (Heterocephalus Glaber). Neuroscience, 230: 56-61, 2013.
  • Mouton PR, et al., Empirical Assessment Of Total Synapse Number In Primate Neocortex. J Neurosci Meth 75: 119-126, 1997.
  • O’Shaughnessy, PJ, et al., Testicular Development In Mice Lacking Receptors For Follicle Stimulating Hormone And Androgen. Plos One. 7:E35136, 2012.
  • Walters, K., et al., No Evidence Of Neurogenesis In Adult Rat Sympathetic Ganglia Following Guanethidine-Induced Neuronal Necrosis, Toxicologic Pathology, In Press.
  • Mouton, PR, et al., Caloric Restriction Attenuates Cortical Amyloidosis In A Double Transgenic Mouse Model Of Alzheimer’s Disease. Neuroscience Lett 464:184-7, 2009.
  • Mouton, PR. Neurostereology, Wiley-Blackwell Press, Boston, 280 Pages, 2014.
  • Weise, C. M., et al., A Post-Mortem Stereological Study Of Striatal Cell Number In Human Obesity. Obesity, 23(1):100-4, 2015.
  • Mouton, PR. Quantitative Anatomy Using Unbiased Stereology, In CRC Handbook Of Imaging In Biological Mechanics (C. Geu And G. Genin, Eds), Taylor & Francis Press, London And New York, 2014.
  • Phoulady, H.A., et al., Automatic Stereology Of Mean Nuclear Size Of Neurons Using An Active Contour Framework. J Chem Neuroanat, 96:110-115, 2019.
  • Perry TA, et al., Pyridoxine-Induced Toxicity In Rats: A Stereological Quantification Of The Sensory Neuropathy. Exp Neurol 190:133-44, 2004.
  • Phoulady, H.A., et al., An Approach For Overlapping Cell Segmentation In Multi-Layer Cervical Cell Volumes. First Place In Overlapping Cervical Cytology Image Segmentation Challenge, IEEE ISBI, Brooklyn, NY, , March 2015.
  • Oaklander, AL, et al. Number Of Langerhans’ Immune Cells In Painful And Non-Painful Human Skin After Shingles. Archives Dermatology Research 294:529-535, 2003.